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Author Topic: florescence vs radioactivity  (Read 8086 times)

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Offline aygroig

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florescence vs radioactivity
« on: January 16, 2006, 03:34:48 AM »
Hi, i 'd like to know if is possible to use  fluorescence methods to substitute the use of radioactivity in the dna, rna analisys.

thank to all

florescence vs radioactivity
« on: January 16, 2006, 03:34:48 AM »

Offline richard03

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florescence vs radioactivity
« Reply #1 on: January 17, 2006, 08:44:55 PM »
What specific method you are using for DNA or RNA analysis? ISH? If this is the case, I am sure you can use fluorescence methods (FISH) to substitute the use of radioactivity.

Richard

Offline ImmunoNYC

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florescence vs radioactivity
« Reply #2 on: January 18, 2006, 12:06:19 AM »
Indeed this is true although expect a decrease in sensitivity which is mostly acceptable especially when probe is good.[/color]

Quote from: "richard03"
What specific method you are using for DNA or RNA analysis? ISH? If this is the case, I am sure you can use fluorescence methods (FISH) to substitute the use of radioactivity.

Richard

florescence vs radioactivity
« Reply #2 on: January 18, 2006, 12:06:19 AM »