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Author Topic: which is more sensitive  (Read 3814 times)

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Offline apple

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which is more sensitive
« on: January 30, 2006, 11:44:08 AM »
Hi,

ICC and IF, which method is more sensitive for staining cytospin slides?

Thanks

which is more sensitive
« on: January 30, 2006, 11:44:08 AM »

Offline ImmunoNYC

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Re: which is more sensitive
« Reply #1 on: January 30, 2006, 11:11:49 PM »
It depends on the protocol of course, but traditionally I would say ICC as usually something like DAB is the chromagen which is very robust and gives an amplified deposition of precipitate in an *enzymatic reaction*. [/color]

Quote from: "apple"
Hi,

ICC and IF, which method is more sensitive for staining cytospin slides?

Thanks

Offline apple

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which is more sensitive
« Reply #2 on: January 31, 2006, 05:33:57 PM »
Yes. I mean comparing ICC (ABC method with DAB kit) and IF (ABC method also), which one is more sensitive?

Offline richard03

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which is more sensitive
« Reply #3 on: January 31, 2006, 06:18:01 PM »
Similar! But I prefer to use IF for cytospin slides and it gives nice appearance.

Quote from: "apple"
Yes. I mean comparing ICC (ABC method with DAB kit) and IF (ABC method also), which one is more sensitive?

Offline ImmunoNYC

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which is more sensitive
« Reply #4 on: January 31, 2006, 07:23:25 PM »
I would still emphatically say DAB as it is based on the enzymatic reaction of HRP and DAB.[/color]

Quote from: "apple"
Yes. I mean comparing ICC (ABC method with DAB kit) and IF (ABC method also), which one is more sensitive?

Offline apple

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which is more sensitive
« Reply #5 on: February 02, 2006, 07:18:28 PM »
I used IF (ABC method) and ICC (ABC method and DAB kit) to stain the same cytospins. I found IF show negative results but ICC show positive results. Is this possible?

Offline ImmunoNYC

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which is more sensitive
« Reply #6 on: February 02, 2006, 08:59:41 PM »
Anything is possible. Assuming your protocols are EXACTLY the same except for DAB vs fluorophore, it is possible if you were just on the cusp of detection limits. I would not expect a robust DAB color though.[/color]

Offline apple

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which is more sensitive
« Reply #7 on: February 03, 2006, 04:41:11 AM »
I used cold acetone fixation for IF and PFA fixation for ICC. IF show negative and ICC show positive. I will use cold acetone for ICC again to double check this.

which is more sensitive
« Reply #7 on: February 03, 2006, 04:41:11 AM »