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Author Topic: For staining cell line, is it possible?  (Read 5847 times)

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Offline apple

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For staining cell line, is it possible?
« on: January 30, 2006, 12:04:01 PM »
Hi,

I stained some cytospin slides (Immunofluorescent method) made from one cell line. I found very interested results. Some cells show positive and some cells don't show positive.
I don't know if this is possible. Because I made the cytospin from pure cell line suspension (100% confluent).  

How to explain my results?

Thanks

For staining cell line, is it possible?
« on: January 30, 2006, 12:04:01 PM »

Offline ImmunoNYC

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For staining cell line, is it possible?
« Reply #1 on: January 30, 2006, 10:59:37 PM »
Possible explanations I can think of:

(1) The staining isn't specific
(2) There are cells in varying stages of the cell cycle so your protein is variably expressed.
(3) Your culture is in the process of "changing" and isn't completely clonal.
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Offline discman

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For staining cell line, is it possible?
« Reply #2 on: January 31, 2006, 06:26:42 AM »
Depending on the antigen that you're looking for, your results are not that surprising. For example, if you're detecting a protein involved in apoptosis, you will get various amounts of positive cells depending for example on the confluency of your culture, nutritional state etc.

In addition, as in Maximina's point (3). Your cell culture is a biological system that is subjected to changes during cultivation. And these changes might result in differences in protein expression, earlier in some cells than in others.

GŁnther

Offline apple

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For staining cell line, is it possible?
« Reply #3 on: January 31, 2006, 05:52:24 PM »
Hi Discman and MaximinaNYC,

Many thanks for your comments.

For staining cell line, is it possible?
« Reply #3 on: January 31, 2006, 05:52:24 PM »