Abcam Ad

Author Topic: Alternative Antigen retrieval techniques??  (Read 5249 times)

0 Members and 1 Guest are viewing this topic.

Offline akleinschmit

  • Newbie
  • *
  • Posts: 4
Alternative Antigen retrieval techniques??
« on: February 03, 2006, 01:17:37 AM »
1.)  I am curious if anyone has experimented with "SIER" (sonication induced epitope retrieval)?  I spotted sonication being used in few articles in the literature, yet I couldn't find a protocol or conditions of use.  Beyond this, does anyone know the mechanism of using sonication with epitope retrieval?

2.)  I also came across DNase being used for epitope retrieval?  Any comments or conditions on/for this enzymes use would be helpful. (dilutant to use, time, temp, etc.)

Alternative Antigen retrieval techniques??
« on: February 03, 2006, 01:17:37 AM »

Offline richard03

  • Administrator
  • GoldMember
  • *****
  • Posts: 1212
Alternative Antigen retrieval techniques??
« Reply #1 on: February 11, 2006, 11:13:55 AM »
I have not done this personally either. This is an interesting method. I found a article using this method and there is detailed method in there. Hope this helps.

Differential Expression of Cyclin D1 in Breast Papillary Carcinomas and Benign Papillomas

Here is another one.

Optimized cyclin D1 immunoperoxidase staining in mantle cell lymphoma

Richard

Alternative Antigen retrieval techniques??
« Reply #1 on: February 11, 2006, 11:13:55 AM »