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Author Topic: 8-ohdg Question  (Read 4716 times)

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Offline Evan

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8-ohdg Question
« on: March 02, 2006, 11:38:19 AM »
So I have been trying to work out a decent stain for 8-ohdg in an oxidative stress model for murine retina, and I was wondering which retrieval method is more appropriate for such a thing - citrate buffer, or proteinase k? The manufacterer recommends proteinase, but the protocol listed here mentions only citrate buffer.

I would think that since 8-ohdg is a nucleic acid stain, one would need to use proteinase k, but any feedback would be appreciated.

8-ohdg Question
« on: March 02, 2006, 11:38:19 AM »

Offline richard03

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8-ohdg Question
« Reply #1 on: March 02, 2006, 12:02:42 PM »
I have used the protocol here and it works well on citrate buffer AR.

Here are some pics using this protocol.

http://ihcworld.com/imagegallery/thumbnails.php?album=search&type=full&search=8-ohdg

Pk may work as some abs work for multiple ARs, but I never tried.

Richard

8-ohdg Question
« Reply #1 on: March 02, 2006, 12:02:42 PM »