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Author Topic: sialyl lewis on carcinoma cells  (Read 4733 times)

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Offline phie

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sialyl lewis on carcinoma cells
« on: March 15, 2006, 06:20:49 AM »
I´m testing whether there are sialyl lewis a & sialyl lewis x on carcinoma cells with ihc.
Right now I work with cultured cells on chamber slides, but somehow it doesn´t really work out. Does anybody have experience with that, for example concerning fixation? Or do you know a better way than cell culture on chamber slides for examination?
Thanks! Sophie

sialyl lewis on carcinoma cells
« on: March 15, 2006, 06:20:49 AM »

Offline richard03

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sialyl lewis on carcinoma cells
« Reply #1 on: April 22, 2006, 02:25:38 PM »
What is your detailed procedure?

You may refer to this article for general information including fixation, etc.

http://www.ihcworld.com/immuncytochemistry.htm

sialyl lewis on carcinoma cells
« Reply #1 on: April 22, 2006, 02:25:38 PM »