Author Topic: Background with tunel  (Read 5951 times)

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Offline Adele

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Background with tunel
« on: March 27, 2006, 03:21:27 PM »
Hello everyone,

I've been doing tunel stainings (using the fluorescein in situ cell death kit from Roche) on Cos7 cells transfected with my molecule of interest, and I am getting intensive background (?) staining which looks like small fluorescent spots in the cytoplasm, but also around the cells. I suspect it has something to do with the transfection agent (Lipofectamine 2000). Did anyone experience anything similar?
Thanks!

Background with tunel
« on: March 27, 2006, 03:21:27 PM »

Offline richard03

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Background with tunel
« Reply #1 on: July 12, 2006, 11:06:24 PM »
Can you post some pictures?

Background with tunel
« Reply #1 on: July 12, 2006, 11:06:24 PM »