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Author Topic: TRS - whats in it?  (Read 6040 times)

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Offline jan21_s

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TRS - whats in it?
« on: April 05, 2006, 02:42:37 AM »
Hi,

can someone tell me whats in Dako's TRS - pH6.0
(Cat no  S1700) - all the data sheet says is that its a "modified citrate buffer".  I suspect that its something in the buffer thats interfering with some of my downstream reactions.  :?

Thanks a lot.

TRS - whats in it?
« on: April 05, 2006, 02:42:37 AM »

Offline richard03

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TRS - whats in it?
« Reply #1 on: April 05, 2006, 09:33:18 PM »
It is probably Tween 20.

Offline jan21_s

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TRS - whats in it?
« Reply #2 on: April 05, 2006, 09:40:47 PM »
Thats what i thought, just hoping that there is no EDTA too...

thanks

Offline ole

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TRS - whats in it?
« Reply #3 on: April 06, 2006, 05:19:33 AM »
Hi
I suspect there is a heat stable enzyme in it, prob very low cons tho. It works very different from std citrate buffer.

It works fine on many antigens that previosly only worked after enzyme treatment. No other retrieval buffers works on these antigens (at least none i have tried).

Citratebuffer have also the ability to chelate Ca, not in the same extent that EDTA-based retrieval buffers tho. So i assume there are no EDTA in it.

Ole

Offline ImmunoNYC

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TRS - whats in it?
« Reply #4 on: April 07, 2006, 12:08:23 PM »
Have you looked at the MSDS it may give you some information. I never asked what specifically was in it as it works so darn well for my immunos I never looked back.[/color]

TRS - whats in it?
« Reply #4 on: April 07, 2006, 12:08:23 PM »