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Author Topic: Brain as a negative control?  (Read 4582 times)

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Offline Cris

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Brain as a negative control?
« on: April 25, 2006, 02:28:36 PM »
Dear All,

Would anybody know a common/easy to work with antibody that would use brain as the negative control tissue?

I am starting to develop a new technique and I work with brain, but I have to show that an non-brain-related antibody treated the same way I do with my brain markers does not stay in the brain as well.

Any help?

Thank a lot!  :D

Cris

Brain as a negative control?
« on: April 25, 2006, 02:28:36 PM »

Offline ImmunoNYC

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Re: Brain as a negative control?
« Reply #1 on: April 26, 2006, 09:47:55 PM »
How about a cytokeratin antibody specific for say gastric or colon epithelium like CK20 or something like that??

Offline Cris

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Will Try It
« Reply #2 on: April 26, 2006, 10:50:15 PM »
I will check on that!
Thank you.
Cris

Offline ImmunoNYC

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Re: Will Try It
« Reply #3 on: April 26, 2006, 11:21:24 PM »
Or you can try a marker of B and T cells such as CD19, CD4, CD8. Although there may be a small number in some of the vessels in your brain section overall they should not stain the brain proper. You can use a spleen or lymph node as positive control/[/color]

Quote from: "Cris"
I will check on that!
Thank you.
Cris

Re: Will Try It
« Reply #3 on: April 26, 2006, 11:21:24 PM »