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Author Topic: staing with unspecific IgG  (Read 2052 times)

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Offline wendy

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staing with unspecific IgG
« on: May 12, 2006, 07:18:35 AM »
Hey!

I have tried staing my negative control with unspecific IgG, and I get a lot of staining. I am working with growth plate cartilage and am using TUF as antogen retrival. I suspect that I have a bad blocking in my section, I use 2% Horse Serum in PBS-tween 0.05% to block. Any suggestion how to make a better blocking. Is it good to combine serum with BSA? And how much BSA to use? 1%?

Thank you for any suggetstions:)

staing with unspecific IgG
« on: May 12, 2006, 07:18:35 AM »

Offline richard03

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staing with unspecific IgG
« Reply #1 on: May 19, 2006, 11:21:12 PM »
The IgG isotype should match your primary antibody, and the conc. should be same as primary antibody as well.

You may want to try PBS negative control to figure out if the unspecific staining is from IgG control.

staing with unspecific IgG
« Reply #1 on: May 19, 2006, 11:21:12 PM »