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Author Topic: IMS and methanol  (Read 5886 times)

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Offline n_bjorkhammer

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IMS and methanol
« on: May 31, 2006, 05:21:43 AM »
Hi all,

Our lab is currently using IMS to fix cytospun slides. These slides are then kept for approximately 6weeks prior to disposal. Occasionally a request for ICC is made and my question is if the fixative for these slides have any implication on the ICC result? The alternative is to airdry the cytospun slides and then to fix them in methanol...

Thanks,

Nick
Niclas Björkhammer, MSc
Technologist II - anatomical Pathology and Morgue
Department of Pathology
Sidra Medical and Research Center
Doha, Qatar

IMS and methanol
« on: May 31, 2006, 05:21:43 AM »

Offline excalibur

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IMS and methanol
« Reply #1 on: May 31, 2006, 10:52:58 AM »
As with any immuno, the antibody determines which fixative is needed.  
Some need methanol, some can use formalin, some need acetone.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline jonnieplumb

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IMS and methanol
« Reply #2 on: June 06, 2006, 10:51:18 AM »
Hi
as mentioned the antibody used will dictate the fixative used. We currently airdry our cytospins and then store them appropriately at -20 until use then you can decide on the fixative you want to use.
Cheers
J

IMS and methanol
« Reply #2 on: June 06, 2006, 10:51:18 AM »