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Author Topic: Cytospin cell loss  (Read 6777 times)

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Offline Pavel

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Cytospin cell loss
« on: July 05, 2006, 07:36:07 PM »
Hi,
I am using a rather old Shandon Cytospin here at our lab. I have heard some comments about it losing cells due to bad balancing.
Anyhow, it has been working for me so far when I was using high cell numbers. Getting into low range I start noticing that when for example my loading estimate is about 40,000 cells I get only about 1,500 cells on the slide. Since I may to spin even fewer cells in the future, I am worrying that none of them may make it  :cry: .
Does any of you having used Cytospin have any experience with cytospinning small cell numbers, or know the normal parameters of cell loss in this technique?

Many thanks ;)
Pavel
UC Davis Cancer Center

Cytospin cell loss
« on: July 05, 2006, 07:36:07 PM »

Offline ImmunoNYC

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Re: Cytospin cell loss
« Reply #1 on: July 05, 2006, 10:35:35 PM »
What speed/times are you using and what kind of cells are you spinning?[/color]

Offline Pavel

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Cytospin cell loss
« Reply #2 on: July 06, 2006, 01:59:56 PM »
White blood cells (a fraction of), spinning for 2 minutes @ 600rpm

Cytospin cell loss
« Reply #2 on: July 06, 2006, 01:59:56 PM »