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Author Topic: flourescent staining for interferon gamma  (Read 1563 times)

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Offline rona

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flourescent staining for interferon gamma
« on: August 08, 2006, 08:30:20 AM »
Hi,

I want to stain interferon-gamma on mouse tissues.
The tissues were fixed with PFA 4% transfered to sucrose 30% (for cryoprotection) and then frozen with LN2/isopentane.  I cut the tissues to 30um.
Can someone reccomend on a protocol to stain the tissues with interferon-gamma?

Thanks,
Rona

flourescent staining for interferon gamma
« on: August 08, 2006, 08:30:20 AM »