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Author Topic: Which markers to be choosed  (Read 4543 times)

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Offline Wang XF

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Which markers to be choosed
« on: August 30, 2006, 12:10:23 PM »
I will demonstrate that fibroblasts can promote angiogenesis in carcinoma.so which markers I should determine ?THX!
mpossible make possible!

Which markers to be choosed
« on: August 30, 2006, 12:10:23 PM »

Offline ghostdog

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Which markers to be choosed
« Reply #1 on: August 31, 2006, 04:43:32 AM »
Hi you could try this, they seem to be fairly specific.

www.acris-antibodies.de/pdf/Focuson0008.pdf

Offline Wang XF

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Which markers to be choosed
« Reply #2 on: September 02, 2006, 01:44:43 PM »
Hi,ghostdog,thank you for your help!
But that is fibroblast-marker antibody ,what I want is myofibroblast`s marker.
mpossible make possible!

Offline ImmunoNYC

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Which markers to be choosed
« Reply #3 on: September 05, 2006, 12:36:36 AM »
How about smooth muscle actin?[/color]

Offline Wang XF

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Which markers to be choosed
« Reply #4 on: September 07, 2006, 08:54:14 AM »
MaximinaNYC, thanks!
a-SMA ,positive in vascular smooth muscle ,fails to purify myofibroblasts from carcinoma. So how I do ???
mpossible make possible!

Offline REDDY

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Which markers to be choosed
« Reply #5 on: October 06, 2006, 05:10:25 PM »
HI WANG,
   WHY DONT YOU USE CATHEPSIN K FOR FIBROBLAST....
-Vasanthi Reddy

Offline ImmunoNYC

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Which markers to be choosed
« Reply #6 on: October 07, 2006, 05:34:47 PM »
Is this really true? I am not so sure.[/color]

Quote from: "Wang XF"
MaximinaNYC, thanks!
a?SMA ?positive in vascular smooth muscle ?fails to purify myofibroblasts from carcinoma. So how I do ???

Offline REDDY

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Which markers to be choosed
« Reply #7 on: October 10, 2006, 05:17:52 PM »
YES...
-Vasanthi Reddy

Offline ImmunoNYC

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Which markers to be choosed
« Reply #8 on: October 11, 2006, 12:06:56 AM »
myofibroblasts in my sections are SMA positive

Offline REDDY

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Which markers to be choosed
« Reply #9 on: October 11, 2006, 11:51:32 AM »
HI,
 I HAVE USED CATHEPSIN K FROM NOVOCASTRA...

http://www.novocastra.co.uk/data/si/cath-k_non_RUO.pdf

WORKED WELL FOR FIBROBLAST....CHECK IT OUT WHETHER IT WILL HELPFUL FOR YOU OR NOT

VASANTHI REDDY
-Vasanthi Reddy

Which markers to be choosed
« Reply #9 on: October 11, 2006, 11:51:32 AM »