Author Topic: Mitochondrial probe for Frozen sections  (Read 4377 times)

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Offline MMehdi

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Mitochondrial probe for Frozen sections
« on: May 18, 2012, 07:18:34 AM »
Hi,

Can anybody suggest me a good mitochondiral marker for Frozen sections Immunohistochemistry?

Thanks

Mitochondrial probe for Frozen sections
« on: May 18, 2012, 07:18:34 AM »

Offline gula

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Re: Mitochondrial probe for Frozen sections
« Reply #1 on: May 18, 2012, 12:08:11 PM »
I can't give you an antibody, but there's a vital-stain of mitochondria with Janusgreen B (0,05% in Ringer). Cover the slide in a wet chamber for 10-20 min. Rinse in water and coverslip with aequous media.
Mitochondria should stain blue-green. The stain needs access to oxygen while staining.
I never did this stain personally. Reference in Romeis.

In Bancroft's the Altmann's technique for mitochondria is described. A stain with Anilin-acid fuchsin on Helly's-fixed paraffin sections.
gula

Re: Mitochondrial probe for Frozen sections
« Reply #1 on: May 18, 2012, 12:08:11 PM »