†††††††††††††††††††††††††††††††††††††

Author Topic: Membrane Antibody!?  (Read 10545 times)

0 Members and 1 Guest are viewing this topic.

Offline figgle

  • Newbie
  • *
  • Posts: 1
Membrane Antibody!?
« on: September 06, 2006, 03:10:22 AM »
Hi,
We made a actin-hCD2 transgenic mice and now I'm confirming hCD2's ubiquitous expression. The problem is that I need a membrane marker to show hCD2 locates on the membrane in different organs, so does anyone have ideas whether there's a membrane antibody showing strong signals on almost all the cells?THX

Membrane Antibody!?
« on: September 06, 2006, 03:10:22 AM »

Offline discman

  • SilverMember
  • *****
  • Posts: 52
Membrane Antibody!?
« Reply #1 on: September 11, 2006, 04:36:29 AM »
Hi all, I'm also interested in an answer to the above question. So, I thought I join in this thread...

We're trying to separate plasma membrane from the rest of the cells. When I was looking for a plasma membrane marker, I found the following site: http://www.organelle-markers.com/plasmamembrane.html

Does anyone around here have any experience with one of those markers? Any help is much appreciated!

GŁnther

Offline richard03

  • Administrator
  • GoldMember
  • *****
  • Posts: 1212
Re: Membrane Antibody!?
« Reply #2 on: September 27, 2007, 07:19:43 PM »
Different organs/cells may need different membrane markers to detect. See review below.

http://www.antibodybeyond.com/reviews/organelle-markers/plasma-membrane-marker.htm

Offline Reo

  • Newbie
  • *
  • Posts: 1
Re: Membrane Antibody!?
« Reply #3 on: October 10, 2007, 02:06:37 PM »
Thank You for this thread, I am also interested in membrane protein markers for colocalisation studies.

I would be especially interested if anyone would now working antibody for plasmatic membrane in plants.

Re: Membrane Antibody!?
« Reply #3 on: October 10, 2007, 02:06:37 PM »