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Author Topic: Formalin fixed cytospun needle washings  (Read 5610 times)

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Offline n_bjorkhammer

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Formalin fixed cytospun needle washings
« on: October 25, 2006, 04:09:52 AM »
Hi,

We recently had a request for IHC on needle washings from a FNA lung. As they arrive in formalin do I;
1. Perform AR since they are formalin fixed
2. Store cytospun slides in methanol, IMS or formalin until such time that they are requried

Can the slides be airdried before being placed in methanol or will this affect the staining quality?

Many thanks
Niclas Björkhammer, MSc
Technologist II - anatomical Pathology and Morgue
Department of Pathology
Sidra Medical and Research Center
Doha, Qatar

Formalin fixed cytospun needle washings
« on: October 25, 2006, 04:09:52 AM »

Offline excalibur

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Formalin fixed cytospun needle washings
« Reply #1 on: October 25, 2006, 05:47:31 PM »
Several options here.

If the cells are suspended in formalin,

1) spin them down, decant the liquid, place a drop of melted agar on a slide, place the bullet of cells in the agar, let cool, remove from slide, and they can be processed and sectioned just like a piece of tissue.

2) Use adhesive slides to do cytospins, since the cells are fixed. Just air dry before staining.

AR depends on which antibody you use and how long the cells were in formalin.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline n_bjorkhammer

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Immunocytochemistry references
« Reply #2 on: October 26, 2006, 04:55:24 AM »
Thanks for the response reg. FF cytospins. Is there any good references on AR on cytology. We are currently using IMS for all our antibodies. I'd like to optimise our Ab as I've d9one with FF paraffin bloicks?

Many thanks

Nick
Niclas Björkhammer, MSc
Technologist II - anatomical Pathology and Morgue
Department of Pathology
Sidra Medical and Research Center
Doha, Qatar

Offline excalibur

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Formalin fixed cytospun needle washings
« Reply #3 on: October 26, 2006, 08:23:36 AM »
Not all antibodies require AR. Some will only work on fresh, unfixed frozen tissue. You will need to check the data sheets of each antibody to see what the company recommends as a staring point.

AR is usually not needed on cytologies, unless they have been fixed for a while.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Formalin fixed cytospun needle washings
« Reply #3 on: October 26, 2006, 08:23:36 AM »