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Author Topic: Bcl1/cyclin D1 protocol  (Read 6479 times)

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Offline maya

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Bcl1/cyclin D1 protocol
« on: December 23, 2006, 10:10:12 AM »
I've started using the ventana system and i can't figure what is the suitable protocol for BCl1/cyclin D1 mAb. I've tried amplifying and using lower Ab dilution but the staining is still very weak.
Does anybody have any sugestions?
thanks
Maya.

Bcl1/cyclin D1 protocol
« on: December 23, 2006, 10:10:12 AM »

Offline dabaicai

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Re: Bcl1/cyclin D1 protocol
« Reply #1 on: March 21, 2009, 07:54:17 PM »
I have got good results from using CyclinD1 antibody (mouse, BD Pharmingen, 1:200) on 4% PFA fixed, paraffin-embedded embryonic brain tissue sections, with regular IHC plus citrate buffer retrieval.
Hh rocks~

Offline gula

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Re: Bcl1/cyclin D1 protocol
« Reply #2 on: March 22, 2009, 04:11:50 AM »
We perform Cyclin D1 (SP4, Neomarker) with CC1 mild, 1:25, 24 min, 37C on the Benchmark XT.
gula

Re: Bcl1/cyclin D1 protocol
« Reply #2 on: March 22, 2009, 04:11:50 AM »