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Author Topic: Receptor labeling  (Read 4884 times)

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Offline Bio

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Receptor labeling
« on: January 19, 2007, 02:12:56 PM »
Internal labeling of phospho-region of a receptor is not working for me. I'm getting signals everywhere in the cell. I'm using 0.2% tween for 10 min to permeabilise the cells.....1% BSA as blocking solution and antibody is diluted in same blocking solution.
Pls. Help ???

Receptor labeling
« on: January 19, 2007, 02:12:56 PM »

Offline richard03

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Re: Receptor labeling
« Reply #1 on: January 19, 2007, 11:40:46 PM »
Your primary antibody concentration maybe too high. Try lower concentration.

Offline ImmunoNYC

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Re: Receptor labeling
« Reply #2 on: February 01, 2007, 11:24:14 PM »
What receptor is it might I ask? How do you know the signal isn't real?

Re: Receptor labeling
« Reply #2 on: February 01, 2007, 11:24:14 PM »