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Author Topic: Burstone's method for Alkaline Phosphatase on frozen section  (Read 2065 times)

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Offline wangl

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Burstone's method for Alkaline Phosphatase on frozen section
« on: January 22, 2007, 08:17:51 PM »
I have a question regarding the  Burstone's method of IHC worldfor Alkaline Phosphatase on frozen sections.

When we follow the procedure on the last step to add aqueos medium to mount the section, the section formed lot of air bubbles. It's very difficult to remove them and therefore, it effected the later analysis. Have you been experience that problem before? Is this caused by Alkaline phosphatase activity? Do you have any solution for the issue?

Thanks for your time :)

Burstone's method for Alkaline Phosphatase on frozen section
« on: January 22, 2007, 08:17:51 PM »

Offline excalibur

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Re: Burstone's method for Alkaline Phosphatase on frozen section
« Reply #1 on: January 23, 2007, 07:58:25 AM »
How experienced are you with coverslipping? You may just need to practice your technique.

Try using the aqueous media by having your slides very wet, place a drop or 2 of the media on the slide, allow the water and media to mix, then place the coverslip on one edge, and slowly lower it. Any air bubbles will rise to the top and you can hold a corner of the coverslip up until they all pop.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
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Re: Burstone's method for Alkaline Phosphatase on frozen section
« Reply #1 on: January 23, 2007, 07:58:25 AM »