Author Topic: How to distinguish between human and mouse cells  (Read 6898 times)

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Offline Marsu

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How to distinguish between human and mouse cells
« on: June 07, 2007, 07:51:42 AM »
Hi,
Does anyone know antibodies to distinguish between human and mouse cells in paraffin sections?
Thanks for your answers.

How to distinguish between human and mouse cells
« on: June 07, 2007, 07:51:42 AM »

Offline excalibur

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Re: How to distinguish between human and mouse cells
« Reply #1 on: June 07, 2007, 10:05:33 AM »
Try an anti-human mitochondrial antibody to see the human cells.
Paula K. Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
8901 S Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3959
www.excaliburpathology.com

Offline ImmunoNYC

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Re: How to distinguish between human and mouse cells
« Reply #2 on: July 31, 2007, 06:35:08 PM »
What kind of cells? Beyond Paula's suggestion above which is precisely correct, you can also stain for mouse or human specific markers if you know what kind of cells you are staining for. Also I suggest you consider haplotype antibodies specific for mouse or human MHC.
« Last Edit: July 31, 2007, 06:37:05 PM by ImmunoNYC »

Offline luisa

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Re: How to distinguish between human and mouse cells
« Reply #3 on: October 17, 2007, 07:28:01 AM »
Does anybody know a good anti-human mitochondria that works on FFPE sections?
Thanks a lot!!!

Offline richard03

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Re: How to distinguish between human and mouse cells
« Reply #4 on: October 17, 2007, 07:47:15 AM »
Use antibody search engine (see link below), use key word "mitochondria" to search. At result page, click refine results "IHC-paraffin". You will find some antibodies that work on paraffin sections.

http://www.antibodybeyond.com/abfinder/absearchengine.htm

Re: How to distinguish between human and mouse cells
« Reply #4 on: October 17, 2007, 07:47:15 AM »