Author Topic: intracellular juxtamembrane protein staining  (Read 9767 times)

0 Members and 1 Guest are viewing this topic.

Offline raoqing

  • Newbie
  • *
  • Posts: 1
intracellular juxtamembrane protein staining
« on: July 12, 2007, 02:15:18 AM »
I want to stain a protein existin in intracellular juxtamembrane.Do I need penetrate the cell membrane before adding antibody.If need, how penetrate it?
Thanks 

intracellular juxtamembrane protein staining
« on: July 12, 2007, 02:15:18 AM »

Offline ImmunoNYC

  • Global Moderator
  • GoldMember
  • *****
  • Posts: 683
Re: intracellular juxtamembrane protein staining
« Reply #1 on: November 14, 2008, 01:36:46 PM »
I assume you are staining cells grown on slides or something similar??? If your antibody is targeted towards an antigen expressed on the inside of the cell (for example the c-terminus of a protein that spans the lipid bilayer) then you would need to permeabilize - especially if your antibody is a monoclonal antibody. Do you know anything about your antibody? Is it polyclonal, monoclonal? If in doubt why not try it on positive control samples with and without permeabilization. 

Re: intracellular juxtamembrane protein staining
« Reply #1 on: November 14, 2008, 01:36:46 PM »