Abcam Ad

Author Topic: BrdU staining and trypsin pre-treatment on cells  (Read 6263 times)

0 Members and 1 Guest are viewing this topic.

Offline MariaKokki

  • Newbie
  • *
  • Posts: 2
BrdU staining and trypsin pre-treatment on cells
« on: July 27, 2007, 02:34:35 PM »
Hi! I want to label a cell line with BrdU and then stain the cells grown on coverslips with anti-BrdU. I will use DAB chromate for staining. According to my antibody's protocol, I need to add trypsin (0.1% in PBS for 20min at 37oC !) before the incubation with BrdU.
Does anybody have any idea of why is that? I am afraid that my cells will detach from the coverslip. Is trypsin pre-treatment really necessary in my case?

BrdU staining and trypsin pre-treatment on cells
« on: July 27, 2007, 02:34:35 PM »

Offline excalibur

  • Global Moderator
  • GoldMember
  • *****
  • Posts: 1109
    • http://www.excaliburpathology.com
Re: BrdU staining and trypsin pre-treatment on cells
« Reply #1 on: July 27, 2007, 06:20:12 PM »
As always, you will just need to try it and see. But cells can be fixed for shorter times than tissues, so trypsin may or maynot be needed. Have you spoken with tech support of the compnay that made your antibody?
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Re: BrdU staining and trypsin pre-treatment on cells
« Reply #1 on: July 27, 2007, 06:20:12 PM »