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Author Topic: SMI32-SMI35  (Read 5637 times)

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Offline Vero36

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SMI32-SMI35
« on: August 21, 2007, 09:20:21 AM »
i work on rat brain and spinal cord.
i already used the SMi35 and SMI32 from Covance on paraffin section (tissues were fixed with 4%PFA)
This is my protocole:
Pretreatment microwave, 10 min in citrate buffer pH6.0
Quentching in NaAzide 20 min
Block with 10% goat serum  20 min
1st Ab dilution 1/500 over night at 4C
2 nd Ab dilution 1/200 ( goat anti  mouse)
HRP  30 min
DAB 2 - 4 min

Could you give some advice about this Ab?

Thanks in advance
Celine

SMI32-SMI35
« on: August 21, 2007, 09:20:21 AM »