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Author Topic: Immunostainings for skin explants  (Read 2593 times)

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Offline Zeev

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Immunostainings for skin explants
« on: November 10, 2007, 08:45:31 AM »
Dear All,

I am a Clinical Researcher in a Medical Device company. IHC is not exactly my field of specialty and I would like to get some expert opinions on the following matter.
Our company is intending to use services from a commercial lab in order to determine effects of our product related to skin rejuvenation, anti ageing, healing activity and angiogenesis.
The following stainings, following treatments with the product, have been suggested:
Basl keratin (CK14)
Collagen III
Collagen IV
Glycosaminoglicans (GAG)
cells in mitosis (KI67)
endothelial cells (PECAM-1)
My questions are:
1. Are all those stainings relevant/necessary for the above mentioned purpose?
2. Are there better suited stainings available?

I am looking forward to getting your expert opinion.

Best regards,

Zeev P.

Immunostainings for skin explants
« on: November 10, 2007, 08:45:31 AM »

Offline ahiscox

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Re: Immunostainings for skin explants
« Reply #1 on: November 13, 2007, 05:35:11 PM »
Some good angiogenesis markers that I've had luck with are Hif-1a, VEGF-A, flt-1, GS-1, VWF, and basic FGF.


Offline richard03

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Re: Immunostainings for skin explants
« Reply #2 on: November 13, 2007, 09:51:56 PM »
CD31 in combination with Smooth Muscle Actin (SMA) are good markers for wound healing in angiogenesis.

Re: Immunostainings for skin explants
« Reply #2 on: November 13, 2007, 09:51:56 PM »