Author Topic: Purification of FITC conjugated antibodies  (Read 4338 times)

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Offline jagan

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Purification of FITC conjugated antibodies
« on: December 20, 2007, 02:10:00 AM »
Dear friends,
What is best column for Purifying FITC Conjugated antibodies other than Protein-Dextrose 10 Column? I am not prefering the 
Protein-Dextrose 10 Column because this column we cant reuse? I tried to wash the column with Equilibration buffer, 3M NaCl and 0.2M NaOH, but no use. Please guide me in this Aspect!

Thanks and Regards,

Jagan

Purification of FITC conjugated antibodies
« on: December 20, 2007, 02:10:00 AM »

Offline ImmunoNYC

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Re: Purification of FITC conjugated antibodies
« Reply #1 on: July 18, 2008, 09:41:32 PM »
Dear friends,
What is best column for Purifying FITC Conjugated antibodies other than Protein-Dextrose 10 Column? I am not prefering the 
Protein-Dextrose 10 Column because this column we cant reuse? I tried to wash the column with Equilibration buffer, 3M NaCl and 0.2M NaOH, but no use. Please guide me in this Aspect!

Thanks and Regards,

Jagan

I use the PD-10 columns but do not reuse. Do you need to reuse?

Re: Purification of FITC conjugated antibodies
« Reply #1 on: July 18, 2008, 09:41:32 PM »