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Author Topic: Formic acid method - basic question  (Read 6522 times)

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Offline kmg47

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Formic acid method - basic question
« on: January 08, 2008, 12:06:11 PM »
Hi all,

I am new to the antigen retrieval process and have a question about the logistics of formic acid treatment.  I made an 88% formic acid solution, but the formic acid remains soluble only when heated...so do I need to keep the slides warm during the incubation process to keep the formic acid liquified, or is it ok to let it solidify on the sections?  Or did I make the original solution incorrectly, and it really should be liquified at room temp?

Thanks for your help!

Formic acid method - basic question
« on: January 08, 2008, 12:06:11 PM »

Offline CanuckPhD

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Re: Formic acid method - basic question
« Reply #1 on: January 09, 2008, 05:42:16 AM »
Formic acid should be a liquid at room temp. It is available as a 88% solution from Sigma and is not that expensive.

Just drop the formic acid onto your slides and then remove at the desired time into water. Caution: do this in a fume hood as the formic acid is caustic and can irritated your eyes.

Good luck

Re: Formic acid method - basic question
« Reply #1 on: January 09, 2008, 05:42:16 AM »