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Author Topic: NBT BCIP and paraffin  (Read 3990 times)

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Offline emanymton

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NBT BCIP and paraffin
« on: February 15, 2008, 04:10:47 AM »
Hello
our colaborator has said that when we leave our tissue (after in toto ISH stain with NBT BCIP) in paraffin all the night the NBT BCIP precipitate after cutting diffuses more than when the tissues are embbed at once.
What do you think about this? ???
Thanks
So sorry for my bad english sure you have understund english is not my tong language.

Ema

NBT BCIP and paraffin
« on: February 15, 2008, 04:10:47 AM »

Offline excalibur

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Re: NBT BCIP and paraffin
« Reply #1 on: February 15, 2008, 09:59:10 AM »
IMHO, no tissue should be left in molten paraffin overnight.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline gula

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Re: NBT BCIP and paraffin
« Reply #2 on: March 21, 2008, 12:21:27 PM »
NBT: literature says: slight lipid solubility, resists organic solvents; red monoformazan and contaminant are lipid and alcohol soluble (Kiernan).

Paraffin and lipids have similarities, and over carried alcohol can solve the contaminant. Perhaps this is your answer.

For routine specimen it is no problem to let the paraffin solidify and warm it again on the next day, if you have no time for embedding. I think it depends on the size of your speciman, what time is needed for infiltration.

gula
« Last Edit: March 21, 2008, 12:33:18 PM by gula »

Re: NBT BCIP and paraffin
« Reply #2 on: March 21, 2008, 12:21:27 PM »