Hi everyone! I'm new on the forum and I have a problem I need your help to solve
I need to make a double IHC, one against a membrane antigen and one against a nuclear antigen
I have three different problems:
1) do I have to use different antigen retrieval techniques? one antigen is unmasked via citrate buffer and heat, while the other one is unmasked with citric acid and heat...
2) if I need to do both, won't heat disrupt my first signal?
3) to reach the nuclear antigen, I'd usually use triton...won't it disrupt my membrane signal?
thank you very much, I really need your help!