Author Topic: immuno EM for Microtubule in vivo  (Read 5846 times)

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Offline foxpeter

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immuno EM for Microtubule in vivo
« on: April 17, 2008, 07:05:43 AM »


I want to observe polymerization of microtubule in vivo.
Cells can't be detached before fixing.
How to detach fixing cells from a dish.

Sincerely

Peter

immuno EM for Microtubule in vivo
« on: April 17, 2008, 07:05:43 AM »

Offline richard03

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Re: immuno EM for Microtubule in vivo
« Reply #1 on: April 18, 2008, 06:26:11 PM »
Use Permanox Dish (specially designed for TEM) to grow your cells so there is no need to worry about detachment.

Re: immuno EM for Microtubule in vivo
« Reply #1 on: April 18, 2008, 06:26:11 PM »