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CD3 should work well with Tris-EDTA pH 9.0 but it may also depend on the antibody source.Most F4/80 antibodies work well with either citrate buffer pH 6 or proteinase K method so the Tris-EDTA may not be the best choice for F4/80 antibody.
Thanks for the explanation! I used Tris-EDTA with 0.05% Tween 20 for antigen retrieval for FFPE mouse spleen. Unfortunately, the test samples were all clean as in absolutely no staining at all! I stained for CD3 (1:200), F4/80 (1:400) and FoxP3 (1:100), which according to the antibody specsheet that these antibodies should work in FFPE tissues. I'm thinking it might be due to the antigen retrieval buffer or the concentration of the antibodies? What are your thoughts?Thanks,Annie