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How can I deparaffinize properly a special TMA section ?

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Offline Yanxia

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strange TMA sections
« on: May 21, 2008, 03:45:38 PM »
 Hi, everyone:

I recently get some paraffin TMA sections from a co-lab. These sections are coated a thick layer of paraffin .So I baked them at 60 degree for one hr, then load to Ventana autostainer . The result showed they were just depaffininized. The second time , baked for 2hrs, the result worst. The thirt time, without baking , directly go to fresh Xylen, 3x30min, 100% ,95%,  ethanol 2x20min, 70% ethanol 1x20min, PBS 3x30 min , then load to autostainer. the result same.
I found the sections seem to have some kind of membrane on them.  It is so frustrating . Does anyone know or has encountered similar problem ?  any suggestions will be grateful. Thanks .

strange TMA sections
« on: May 21, 2008, 03:45:38 PM »

Offline richard03

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Re: strange TMA sections
« Reply #1 on: May 22, 2008, 08:56:58 PM »
Some people use Tape Transfer method to cut and collect TMA sections. The Tape Transfer slides were coated with a layer of adhesive film. That might be the case. Ask the company where you got the TMA slides.

Offline Yanxia

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Re: strange TMA sections
« Reply #2 on: May 23, 2008, 02:06:29 PM »
Hi, richard:
thank you very much for your advice. I think that is the problem . I will check the co-lab.

Re: strange TMA sections
« Reply #2 on: May 23, 2008, 02:06:29 PM »