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Author Topic: labelling cells on slides  (Read 5414 times)

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Offline pweston

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labelling cells on slides
« on: June 18, 2008, 10:06:00 AM »
I have been given some cells to label with an antibody.  They are formalin fixed in a microfuge tube.  I usually embed everything in paraffin, or, in the case of EM, plastic .  Can I just smear these cells on a charged slide somehow and then label with the antibody?  I have never done this before.

Does anyone have a protocol for this?

Thanks!

labelling cells on slides
« on: June 18, 2008, 10:06:00 AM »

Offline excalibur

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Re: labelling cells on slides
« Reply #1 on: June 18, 2008, 12:06:29 PM »
You can process them to paraffin as a cell block. I don't recommend just smearing them on a slide if they are already fied. They will not stick good.

Spin the tube down to make a pellet, decant the supernant, place the pellet in biopsy paper folded so no cells are lost, put in a cassette, and process like a tissue block.

If you don't have access to a centrifuge, simply filter into the biopsy paper folded like filter paper.
Paula Keene Pierce, HTL(ASCP)HT
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Offline ImmunoNYC

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Re: labelling cells on slides
« Reply #2 on: January 19, 2009, 04:15:10 PM »
Agree with the suggestion to make a cell block. This can be done for paraffin or cryosections. Just do it to whatever format works best with your antibody protocol.

Another alternative is cytospin.

Re: labelling cells on slides
« Reply #2 on: January 19, 2009, 04:15:10 PM »