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Author Topic: Xylene Substitute freshness  (Read 4016 times)

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Offline Rocky E

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Xylene Substitute freshness
« on: June 23, 2008, 12:13:48 PM »
Hi,

Our lab is just starting to use IHC again, and we seem to be going through a lot of Xylene Substitute.  In an effort to cut back, we are trying to figure out how long a dip tray full of Xylene Subsitute can be used before it needs to be replaced with a fresh batch.  Anyone have any idea?  Thanks.


Xylene Substitute freshness
« on: June 23, 2008, 12:13:48 PM »

Offline CanuckPhD

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Re: Xylene Substitute freshness
« Reply #1 on: June 24, 2008, 01:42:07 AM »
Our lab does 300 sections then rotates the xylenes and alcohols. This seems to work well for us. Of course if someone contaminates the xylene with water then all have to be changed. This is the same for the substitutes that I have worked with (though I find nothings works as well as xylene)

Offline excalibur

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Re: Xylene Substitute freshness
« Reply #2 on: June 24, 2008, 09:12:12 AM »
I agree with CanuckPhD.

I use substitute to process, but xylene to deparaffinize and clear on the stainer.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline richard03

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Re: Xylene Substitute freshness
« Reply #3 on: June 24, 2008, 09:27:18 PM »
I have to agree with both CanuckPhD and excalibur.

Keep in mind that if you stain 25-50 um thick sections, xylene/subsitute needs to be changed more frequently.
   

Offline iLOVEihc

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Re: Xylene Substitute freshness
« Reply #4 on: March 01, 2013, 11:06:43 AM »
I don't like xylene because it's a toxic solution. I was thinking of using either:
1) Histoclear which is distilled from oranges (so is obviously a limonene). I have read that it doesn't mix well with xylene hence problems above.
2) Histochoice by Amresco (which i have no idea what is in it!) Any ideas? I think it is some kind of aliphatic.

Are aliphatics better than limonenes as substitutes? but more toxic?

I will mount with DPX. 



IHClovER

Re: Xylene Substitute freshness
« Reply #4 on: March 01, 2013, 11:06:43 AM »