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Author Topic: Milli-Q water pH?  (Read 25707 times)

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Offline voland

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Milli-Q water pH?
« on: July 22, 2008, 09:38:50 AM »
Hello,

I was wandering what the pH of Milli-Q water should be. My measurements show ph 8,6! And the PBS that I get has a pH from 7,46 to 7,55. Could this affect my results during IHC. Lately I have some tissues being destroyed and I dont know why. Also another person at the lab had the same problem and we did not use the same reagents, only the water! What do you think?

Milli-Q water pH?
« on: July 22, 2008, 09:38:50 AM »

Offline richard03

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Re: Milli-Q water pH?
« Reply #1 on: July 22, 2008, 07:16:00 PM »
As long as your PBS or washing buffer pH is within the range required (i.e. pH7.2-7.4), It should not affect the IHC result. But high pH water tends to damage tissue section so try NOT to use only water for washing and diluting.

Offline ASluo

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Re: Milli-Q water pH?
« Reply #2 on: July 26, 2008, 11:16:48 PM »
Some of our colleagues experienced problems (tissue sections coming off the slides) when they use Milli-Q water to water to wash off the substrate. When they substitute that with normal tap water, the problem was solved!

Annie :)

Offline excalibur

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Re: Milli-Q water pH?
« Reply #3 on: July 27, 2008, 11:07:48 AM »
Yep, tap water contains "buffers".
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline banty

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Re: Milli-Q water pH?
« Reply #4 on: April 15, 2010, 08:25:18 AM »
Can anybody tell me the pH of Milli Q water. The water I analyzed show readings between 6.8-6.6 but I want to conform.
Thanks
Regards

Offline Yao

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Re: Milli-Q water pH?
« Reply #5 on: May 28, 2010, 09:42:43 AM »
Can anybody tell me the pH of Milli Q water. The water I analyzed show readings between 6.8-6.6 but I want to conform.
Thanks
Regards

our millQ water is more basic than yours, but if the PBS is within normal PH range, i think you don't need to concern this.

Re: Milli-Q water pH?
« Reply #5 on: May 28, 2010, 09:42:43 AM »