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Author Topic: Detection of membrane staining  (Read 5777 times)

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Offline smopro

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Detection of membrane staining
« on: July 29, 2008, 03:43:26 PM »
Hi, I'm labelling pfa-fixed cells with Ab and use colorimetric (hrp-DAB) detection. The Ag is extracellular, membrane bound. Upon looking at the cells under a light microscope, should I expect to see a predominent membrane staining or the staining will appear all over the cell. In other words, would a IF/confocal be a better method at detecting this Ag?

Any comments, thanks so much

Detection of membrane staining
« on: July 29, 2008, 03:43:26 PM »

Offline richard03

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Re: Detection of membrane staining
« Reply #1 on: July 29, 2008, 09:31:09 PM »
With colormetric detection, you will most likely see positive staining all over the cell. IF/confocal is better approach.

Offline ImmunoNYC

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Re: Detection of membrane staining
« Reply #2 on: January 19, 2009, 04:06:09 PM »
It would also appear all over the cell as the cell has a membrane ontop of it when plated out, do you know what I mean?

Also PFA can cause some permeabilization which could confuse the issue with IHC or IF.

Re: Detection of membrane staining
« Reply #2 on: January 19, 2009, 04:06:09 PM »