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Author Topic: Organelle Fixation Protocols  (Read 10190 times)

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Offline science

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Organelle Fixation Protocols
« on: October 13, 2008, 04:26:09 PM »
Is there a chart that describes what fixation methods work best for various organelles? What works well for lysosomes?

Organelle Fixation Protocols
« on: October 13, 2008, 04:26:09 PM »

Offline richard03

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Re: Organelle Fixation Protocols
« Reply #1 on: October 13, 2008, 11:04:10 PM »
In general, formalin or formaldehyde is the best fixative for preserving tissue and cell morphology so it should work well for various organelles.

Offline science

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Re: Organelle Fixation Protocols
« Reply #2 on: October 14, 2008, 12:18:35 AM »
where can I find a good formalin fixation protocol?

Offline richard03

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Re: Organelle Fixation Protocols
« Reply #3 on: October 14, 2008, 07:04:41 AM »
You can buy a bottle of formaldehyde and dilute 1:10 with water. Here is a protocol list for your reference.

http://www.ihcworld.com/_protocols/histology/fixatives.htm

Offline monia

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Re: Organelle Fixation Protocols
« Reply #4 on: February 28, 2009, 01:08:13 PM »
Effect of fixation protocols on muscle preservation and ... relative organelle
volumes is fairly insensitive to variations in preparative ...

Offline ImmunoNYC

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Re: Organelle Fixation Protocols
« Reply #5 on: March 06, 2009, 11:39:54 PM »
Ruchard why don't you suggest to dilute in PBS which is what I would do? Just curious ...

Offline richard03

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Re: Organelle Fixation Protocols
« Reply #6 on: March 07, 2009, 02:32:08 PM »
That was just protocol I have been using. But I agree that Buffered Formalin should work better than Un-buffered formalin, especially in preserving ultrastructure.

Ruchard why don't you suggest to dilute in PBS which is what I would do? Just curious ...

Re: Organelle Fixation Protocols
« Reply #6 on: March 07, 2009, 02:32:08 PM »