Author Topic: Microglia Markers  (Read 37039 times)

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Offline voland

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Microglia Markers
« on: October 21, 2008, 11:12:05 AM »
Hi,

 I am quite confused. What is the best and most specific marker for microglia? CD11b, lectin, CD68, Iba1 other? I want to stain rat spinal cord and brain and do quantification analysis between 2 different groups of rats. What is best in order to study if there is a difference in microglia activation.

Thanks!

Microglia Markers
« on: October 21, 2008, 11:12:05 AM »

Offline richard03

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Re: Microglia Markers
« Reply #1 on: October 21, 2008, 08:56:17 PM »
I have used this protocol and it worked well.

http://www.ihcworld.com/_protocols/special_stains/microglia.htm

Offline dutchmaninvienna

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Re: Microglia Markers
« Reply #2 on: October 23, 2008, 08:42:22 AM »
For activation of microglial cells I would use the ab ED1, which recognises a CD68 like molecule in rat. If you use markers like CD11b or iba-1 you will stain both resting as well as activated microglia.

jan
Jan Bauer, PhD
Associate Professor
Div. of Neuroimmunology
Center for Brain Research
Medical University of Vienna, Austria

HertyGytro

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Re: Microglia Markers
« Reply #3 on: December 14, 2008, 04:22:04 AM »

Re: Microglia Markers
« Reply #3 on: December 14, 2008, 04:22:04 AM »