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Author Topic: Help: for longitude intestine  (Read 2923 times)

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Offline xiaomao_470

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Help: for longitude intestine
« on: October 31, 2008, 12:40:34 PM »
Hi, I am a beginner for dissection mouse tissues. I am trying to get longitude intestine but found quite tricky. Does someone know how to dissect the gut and get nice longitude structure. Cheers.

Help: for longitude intestine
« on: October 31, 2008, 12:40:34 PM »

Offline Cardio

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Re: Help: for longitude intestine
« Reply #1 on: October 31, 2008, 01:39:07 PM »
Here is how I would do it for frozen sections. Sandwich the tissue between 2 slides with your freezing medium (you may want to use other slides as spacers to keep the natural thickness of the gut). Then freeze the tissue with cytofreeze or by some other method. Once frozen pull apart one slide. If it doesn't come apart easily warm up the slide with your thumb.

Next place the slide with the tissue on a cast of freezing medium (OTC). Then pull off the other slide.

If by chance a little tissue comes off with the slide then use silicone grease on the slides before you build the sandwich. Make sure you just use a thin film of grease. Silicone grease has no autoflouresence.

Re: Help: for longitude intestine
« Reply #1 on: October 31, 2008, 01:39:07 PM »