General Research Topics > Microbiology

Gene knockdown in bacteria


Hi Everbody
my question about How can I insert SiRNA in gram negative bacteria?
In Eukaryote cell by transfectamin is possible but in prokaryote cell(bacteria) is impossible
please let me methods os insertion SiRNA in bacteria

So not my forte but would regular transformation work?

I know nothing about bacteria but are the mechanics of RNAi conserved in bacteria? Have you looked in literature, someone must have done what you are trying to do?

Tracking the source of this article might be of help to you. It discuses how pro-siRNAs—short for prokaryotic siRNAs are introduced into Bacteria.


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