Author Topic: Gene knockdown in bacteria  (Read 13859 times)

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Offline imani_a

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Gene knockdown in bacteria
« on: December 02, 2008, 12:08:24 AM »
Hi Everbody
my question about How can I insert SiRNA in gram negative bacteria?
In Eukaryote cell by transfectamin is possible but in prokaryote cell(bacteria) is impossible
please let me methods os insertion SiRNA in bacteria

Gene knockdown in bacteria
« on: December 02, 2008, 12:08:24 AM »

Offline ImmunoNYC

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Re: Gene knockdown in bacteria
« Reply #1 on: February 14, 2009, 01:34:06 PM »
So not my forte but would regular transformation work?

Offline ImmunoNYC

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Re: Gene knockdown in bacteria
« Reply #2 on: February 14, 2009, 01:36:19 PM »
I know nothing about bacteria but are the mechanics of RNAi conserved in bacteria? Have you looked in literature, someone must have done what you are trying to do?

Offline MMaggie

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Re: Gene knockdown in bacteria
« Reply #3 on: January 16, 2017, 07:50:36 AM »
Tracking the source of this article might be of help to you. It discuses how pro-siRNAs—short for prokaryotic siRNAs are introduced into Bacteria.

https://vector.childrenshospital.org/2013/03/rna-interference-putting-bacteria-to-work-to-silence-genes/
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Re: Gene knockdown in bacteria
« Reply #3 on: January 16, 2017, 07:50:36 AM »