Author Topic: collagen1 for use in mouse kidney  (Read 2425 times)

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Offline adndoove

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collagen1 for use in mouse kidney
« on: January 16, 2009, 05:29:37 AM »
Hello, Has anybody got experience with staining for collagen1 in the kidney of mice?
I need a good immuno which is also capable of staining collagen producing cells (myofibroblasts emerging after ureter obstruction).
The antibocy I tested so far is from abcam (ab21286), but it is no use. It gives a great deal of background, mainly in tubuli, while not staining the interstitium.
I tried pepsin, citrate and no antigen retrieval, 90 min and overnight incubation but nothing worked.
Would be great if you could help me, thx!
Amélie (Utrecht)

collagen1 for use in mouse kidney
« on: January 16, 2009, 05:29:37 AM »

Offline richard03

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Re: collagen1 for use in mouse kidney
« Reply #1 on: January 17, 2009, 02:19:56 PM »
The background maybe due to endogenous biotin (Kidney usually contains very intense endogenous biotin) so you may want to try "avidin/biotin block" if you haven't done so. It may work better if the antibody is diluted more.


Offline ImmunoNYC

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Re: collagen1 for use in mouse kidney
« Reply #2 on: January 28, 2009, 02:52:48 PM »
I agree with Richard. Likely biotin background.

Re: collagen1 for use in mouse kidney
« Reply #2 on: January 28, 2009, 02:52:48 PM »