I am not sure whether this is the best category to post my question, but I try.
I am quite new to IHC and I have used only the free floating sections technique using wells-plates to perform the immunostaining.
Precisely I use 6-wells culture cell plates with the according well-inserts with membrane.
And I used to re-use these wells-plates (and the inserts) after staining! I let them overnight in a bath of cleaning solution (the same as for the cleaning of lab dishes), then I rinse them with tap water & finally let them in a bath of deionized water overnight.
However, I recently read a protocol which warns not to re-use the wells-plates for staining. Only the plates for the PBS washes could be re-used but after a more complicated cleaning procedure than mine.
What do you do/advise? If you do re-use the wells-plates, how do you clean them?
Thank you very much in advance for your help!