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Author Topic: How long will fixed cells keep?  (Read 9331 times)

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Offline Bastet13

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How long will fixed cells keep?
« on: March 10, 2009, 11:04:08 AM »
Hi,

I have just started doing some ICC on SMCs, and was wondering if anyone knows if there is a way to fix multiple slides of cells and keep them for later usage.  I am currently using acetone (8 mins) for fixation.

Thanks!

Sara

How long will fixed cells keep?
« on: March 10, 2009, 11:04:08 AM »

Offline ImmunoNYC

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Re: How long will fixed cells keep?
« Reply #1 on: March 11, 2009, 10:45:45 AM »
I fix my SMC and EC monolayers in 4% PFA for 15 minutes and keep in PBS for weeks sometimes before staining. If you fix in acetone you can dry and store indefinitely at -80 deg C.

Offline Bastet13

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Re: How long will fixed cells keep?
« Reply #2 on: March 18, 2009, 11:11:41 AM »
Thanks so much for your help, it's greatly appreciated!

Re: How long will fixed cells keep?
« Reply #2 on: March 18, 2009, 11:11:41 AM »