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Author Topic: MLH1 and MSH2  (Read 9379 times)

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Offline Ruth

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MLH1 and MSH2
« on: September 27, 2004, 05:20:55 PM »
Hi,

I am trying to do the staining of anti-MLH1  and  anti-MSH2 paraffin section.
I would like to known what the better clone and protocol for MLH1 and MSH2
Thanks Ruth

MLH1 and MSH2
« on: September 27, 2004, 05:20:55 PM »

Offline richard03

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MLH1 and MSH2
« Reply #1 on: September 27, 2004, 08:54:01 PM »
MLH1 (clone 14) and MSH2 (clone FE11) both work with paraffin sections.

Offline Ruth

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MLH1 and MSH2
« Reply #2 on: September 28, 2004, 03:55:56 PM »
ThanKs

But who comercialize this antibodies?

Zymed, Pharmigen???

Gracias...
Ruth

Offline richard03

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MLH1 and MSH2
« Reply #3 on: September 28, 2004, 04:01:17 PM »
Yes. Zymed has these antibodies.

Offline Lizzy

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MLH1 and MSH2
« Reply #4 on: January 20, 2006, 03:41:43 AM »
I  have been having some trouble optimising the staining of MLH-1.  The anibody seems to be very weak.  We use DAKO ph9 retrieval solution in a pascal pressure cooker 3 mins at 119C, a Dako cytomation automatic stainer.  Does anyone have any success using other staining methods?

Offline ole

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MLH1 and MSH2
« Reply #5 on: January 20, 2006, 07:41:17 AM »
hi Lizzy & Ruth
Your retrieval seems fine Lizzy.
I have tried the zymed ab. Got better (i must say wonderful :wink: ) results using these protocols.

http://www.immunoportal.com/modules.php?name=Recipes&op=viewrecipe&recipeid=173&offset=10#start

http://www.immunoportal.com/modules.php?name=Recipes&op=viewrecipe&recipeid=228&offset=12#start

Ole

MLH1 and MSH2
« Reply #5 on: January 20, 2006, 07:41:17 AM »