Author Topic: Choosing Protease for AR  (Read 4766 times)

0 Members and 1 Guest are viewing this topic.

Offline thomas_leeyl

  • HighTech
  • ***
  • Posts: 18
Choosing Protease for AR
« on: May 18, 2009, 11:49:14 PM »
which type of protease i should choose to do the antigen retrieval on paraffin embedded section? there are so many choices from commercial company. what criteria should i need to consider when i choose it? any protocol suggested?

Choosing Protease for AR
« on: May 18, 2009, 11:49:14 PM »

Offline richard03

  • Administrator
  • GoldMember
  • *****
  • Posts: 1212
Re: Choosing Protease for AR
« Reply #1 on: May 19, 2009, 11:28:58 PM »
Proteinase K is commonly used for AR. It works for most of time when protein digestion is needed. Refer to this protocol.

http://www.ihcworld.com/_protocols/epitope_retrieval/proteinase-k.htm

Re: Choosing Protease for AR
« Reply #1 on: May 19, 2009, 11:28:58 PM »