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what is the most appropriate method to fix smear of fine needle aspirate?

10%formalin
0 (0%)
acetone:methanol
0 (0%)
air dry
0 (0%)
ethanol
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acetone
0 (0%)

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Author Topic: fixation of fine needle aspirate smears  (Read 7505 times)

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Offline naturedream

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fixation of fine needle aspirate smears
« on: July 27, 2009, 12:56:15 PM »
i am doing icc on aspirate smears of lymph node, my problem is proper fixation.
i would like to know how to start with aspirate smears?? ???

fixation of fine needle aspirate smears
« on: July 27, 2009, 12:56:15 PM »

Offline excalibur

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Re: fixation of fine needle aspirate smears
« Reply #1 on: July 28, 2009, 07:48:06 AM »
This can depend on what antibody you are using. Most commonly,the smears are air dried and then fixed with what is recommended on the antibody data sheet.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
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www.excaliburpathology.com

Offline naturedream

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Re: fixation of fine needle aspirate smears
« Reply #2 on: July 28, 2009, 12:51:57 PM »
thanks for your reply,


Offline EvanStark

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Re: fixation of fine needle aspirate smears
« Reply #3 on: January 19, 2010, 06:57:47 AM »
This can depend on what antibody you are using. Most commonly,the smears are air dried and then fixed with what is recommended on the antibody data sheet.

Thanks for clearing this up excalibur. I thought smears were usually air dried but wasn't too sure.

I think that Phen375 is pretty good like this http://skinnyexpress.com/phen375-review post says.
« Last Edit: November 16, 2015, 12:02:28 PM by EvanStark »

Re: fixation of fine needle aspirate smears
« Reply #3 on: January 19, 2010, 06:57:47 AM »