Author Topic: deparaffin block  (Read 6907 times)

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Offline Kahn IG 88

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deparaffin block
« on: August 19, 2009, 11:08:00 AM »
I am looking to deparaffin the sample i already have in a block and get it back to water.  What is the best way to go about this?

deparaffin block
« on: August 19, 2009, 11:08:00 AM »

Offline CanuckPhD

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Re: deparaffin block
« Reply #1 on: August 20, 2009, 02:06:43 AM »
Cut away as much paraffin as possible, place on warm plate to melt paraffin then place in xylene for 2 hours and then repeat xylene bath 2X with 1 hour incubations. Then 2X 100% alcohol, 2X95%, 2X 80% and then 1X50% all for 1 hour. Then place in water. This is based on normal sized tissue pieces, if you are doing large tissues then the times must be increased.

Why do you want to do this? If it is because the original fixation wasn't good then this won't help. We only do this if there were issues in the processing (example someone mixed up the xylene and alcohol solutions on the processor).

Offline Kahn IG 88

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Re: deparaffin block
« Reply #2 on: August 25, 2009, 10:16:30 AM »
someone wants to get some dna from the samples.  Thank you, this should work nicely.

Offline CanuckPhD

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Re: deparaffin block
« Reply #3 on: August 26, 2009, 02:39:59 AM »
If they want DNA from the samples then I think cutting some sections, placing them in eppendorf tubes and using xylene to remove the paraffin, followed by alcohol and water should work. That way your block stays intact and it is faster.


Re: deparaffin block
« Reply #3 on: August 26, 2009, 02:39:59 AM »