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Author Topic: Immunostaining  (Read 3464 times)

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Offline Lee

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Immunostaining
« on: November 09, 2009, 02:05:25 PM »
Can frozen tissue that has been immunostained be immunostained again with a different antibody after the first immunostaining is finished?  If so, what steps need to be taken?

Immunostaining
« on: November 09, 2009, 02:05:25 PM »

Offline excalibur

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Re: Immunostaining
« Reply #1 on: November 11, 2009, 11:08:58 AM »
Yes it can. How did you detect the first antibody? DAB, fluor? Take care in removing any coverslip and staining, as tissue may wash off the slide.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline Lee

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Re: Immunostaining
« Reply #2 on: November 11, 2009, 11:17:54 AM »
It was DAB.  Coverslip hadn't even completely dried so removal was easy.  I tried it and it seemed to work.  Thanks!

Re: Immunostaining
« Reply #2 on: November 11, 2009, 11:17:54 AM »