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Author Topic: Storing whole mouse brain  (Read 3251 times)

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Offline biswa

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Storing whole mouse brain
« on: December 01, 2009, 06:09:54 AM »
Hello all, I am new to this forum and this is my first post. I wish to purify one protein form mouse brain. But unfortunately I do not have all the reagents to prepare buffer needed for making brain homogenate. Is that possible for me to preserve the whole brain for future use without compromising its molecular integrity. I do not have any expertise or knowledge in this field. I would highly appreciate responses in this regard.
With thanks
Biswanath

Storing whole mouse brain
« on: December 01, 2009, 06:09:54 AM »

Offline CanuckPhD

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Re: Storing whole mouse brain
« Reply #1 on: December 01, 2009, 12:15:39 PM »
For protein isolation we dissect out the areas that we are interested in (example hippocampus, cerebellum, cortex etc) at the time of killing. These areas are then snap frozen in liquid nitrogen and stored at -80 until we want to isolate the protein. If we are not interested in specific areas we just bisect the brain and then freeze in liquid nitrogen.

We don't have any major issues with this protocol. Of course it is best to compare fresh brain and stored brain for your protein of interest as all protocols do not serve for all conditions.

Good luck.

Re: Storing whole mouse brain
« Reply #1 on: December 01, 2009, 12:15:39 PM »